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期刊论文 67

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关键词

CP）；符号间干扰（inter symbol interference， ISI）；载波间干扰（inter carrier interference 1

DNA 1

ICI）；最大似然估计（maximum likelihood estimation 1

MLE） 1

OFDM）；快速傅立叶变换（Fast Fourier transform， FFT）；循环前缀（cyclic prefix 1

RNA干扰（RNAi） 1

SARS-CoV-2干扰 1

Z干扰信道；非正则信号；和速率；帕累托边界；协方差；伪协方差 1

circRNA 1

microRNA(miRNA) 1

传输性能分析模型 1

佩戴口罩 1

信使核糖核酸（mRNA）疫苗 1

信息流分析；业务流程执行语言；Petri网；干扰性 1

分子机制 1

地下无线电通信 1

基于RNA的生物防治 1

基于质谱的免疫肽组学 1

多用户干扰 1

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Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene

Zhengjuan LIU, Jie BIAN, Yuchuan WANG, Yongli ZHAO, Dong YAN, Xiaoxia WANG

《医学前沿（英文）》 2009年 第3卷 第1期   页码 57-60 doi: 10.1007/s11684-009-0003-z

摘要： Leptin resistance is a main mechanism of acquired childhood obesity, and the suppression of long form of leptin receptor (OBRb) gene expression in diet-induced obese rats indicates that the down-regulation of OBRb gene expression plays a pivotal role in the mechanism of leptin resistance. The aim of the present study was to construct the lentiviral RNA interference (RNAi) vector of rat OBRb gene and evaluate the effects of siRNA on silencing OBRb gene expression. The target sequence of siRNA-OBRb was designed, and the complementary DNA containing both sense and antisense oligonucleotides was synthesized. After phosphorylation and annealing, these double-stranded DNA was cloned to pRNA-lentivector-VGFP to construct pRNA-Lenti-OBRb-VGFP recombinants with U6-containing promoter, target sequence and Poly III terminator. Then, the products were confirmed by electrophoresis and sequencing analysis, and the effects of RNAi on reducing gene expression were further confirmed by real-time polymerase chain reaction in transfected rat glioma cells expressing OBRb. The target sequence of siRNA-OBRb was successfully cloned to pRNA-lentivector-VGFP, and the RNAi protocol specifically reduced the expression of OBRb mRNA by approximately 80% compared with controls in transfected rat glioma cells. The successful construction of rat lentivirus vectors expressing OBRb-specific shRNA may be useful for further investigation .

关键词： receptors     leptin     RNA interference     lentivirus vector    

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Effects of RNA interference targeting angiotensin 1a receptor on blood pressure and cardiac hypertrophy

ZHANG Jingqun, SUN Honglei, MA Yexin, WANG Daowen

《医学前沿（英文）》 2008年 第2卷 第1期   页码 19-24 doi: 10.1007/s11684-008-0005-2

摘要： The aim of this study is to investigate the effects of RNA interference (RNAi) targeting angiotensin 1a receptor (AT1a) on blood pressure and cardiac hypertrophy of rats with renovascular hypertension. Two RNAi plasmids, pAT1a-shRNA1 and pAT1a-shRNA2 each carrying a U6 promoter and an AT1a-specific shRNA-coding template sequence corresponding to the sites 928–946, 978–996 of the mRNA transcript, and a control plasmid pCon carrying a nonspecific shRNA-coding sequence were constructed. Thirty Sprague – Dawley rats with renovascular hypertension (2-kidney 1-clip) were randomly divided into 5 equal groups: Control group (without any intervention), pAT1a-shRNA1, pAT1a-shRNA2, pCon groups (with injection of the corresponding plasmid 4 mg/kg respectively into the tail vein), and valsartan group (30 mg/kg·d by gavage). Three weeks after drug administration, pAT1a-shRNA1, pAT1a-shRNA2 and valsartan respectively resulted in decrease of the tail blood pressure by (15.1 ± 5.4), (16.4 ± 8.4) and (30.6 ± 18.2) mmHg. However, the tail blood pressure increased further by about 25 mmHg in both of pCon and control groups. The carotid artery pressures of pAT1a-shRNA1, pAT1a-shRNA2 and valsartan groups were all significantly lower than those of the control and pCon groups. The ratio of left ventricle weight to body weight (LV/BW) of the rats in pAT1a-shRNA1, pAT1a-shRNA2, and valsartan groups decreased significantly than in the control group ( < 0.01), similar to those of the normal SD rats( > 0.05). Histopathological examination showed that the myocardiocytes were significantly hypertrophic and the basal membrane of the aorta was significantly thickened in the control group and such changes were alleviated in the pAT1a-shRNA1, pAT1a-shRNA2 and valsartan groups. Compared with the control group, pAT1a-shRNA1 and pAT1a-shRNA2 groups had lowered expression of AT1 receptor (in the myocardium and the thoracic aorta (all < 0.01); however, there were no significant differences in expression levels of AT1 receptor in valsartan and the control groups ( > 0.05). We conclude that RNAi targeting AT1a receptor inhibits the development of renovascular hypertension and the accompanying cardiac hypertrophy. RNAi technology may become a new strategy of gene therapy for hypertension.

关键词： therapy     Sprague     administration     cardiac hypertrophy     valsartan    

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基于RNA的生物防治——一种作物保护新模式 Review

Matthew Bramlett, Geert Plaetinck, Peter Maienfisch

《工程（英文）》 2020年 第6卷 第5期   页码 522-527 doi: 10.1016/j.eng.2019.09.008

摘要： 在过去的几年中，RNA干扰（RNAi）过程被认为是一种非常有前景的新方法，可作为化学和生物害虫防治剂、植物保护剂等叶面喷施、土壤或种子处理的补充。基于RNA的活性成分（AI）具有独特的作用方式，可以通过基因修饰（GM）和生物防治两种途径来实现。由于基于RNA的AI可利用自然过程来发挥控制作用，同时它们具有高度选择性，降低了非目标生物（NTO）的风险，因此基于RNA的AI有望提供未来作物保护剂所需要的选择性和可持续性。本文讨论了基于RNA的生物防治的替代方案在作物保护中的优势和局限性，以及RNA生物防治科罗拉多马铃薯甲虫（CPB）、玉米根虫（CRW）和大豆臭虫（SSB）的最新研究进展。在实现各种基于RNA的产品及其广泛使用和应用的道路上，仍然存在许多挑战。尽管如此，我们仍可预期到，基于RNA的AI将成为有价值的新工具，以补充当前的农作物保护解决方案。

关键词： 基于RNA的生物防治     RNA干扰（RNAi）     科罗拉多马铃薯甲虫（CPB）     玉米根虫（CRW）     大豆臭虫（SSB）    

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Gene silencing efficiency of shRNA expression vectors targeting Cx43

Cuihong ZHENG, Yunxia WU, Guangying HUANG, Wei WANG

《医学前沿（英文）》 2009年 第3卷 第2期   页码 130-135 doi: 10.1007/s11684-009-0030-9

摘要： Our previous studies showed that there were close relationships between connexin 43 (Cx43) and acupoints and meridians. In order to further investigate the effect of Cx43 in acupuncture treatment, RNA interference technique was used to construct small hairpin RNA (shRNA) expression vectors targeting Cx43 and identify the efficiency of RNA interference in NIH/3T3 cell lines for further use . Aiming directly at the two targets of Cx43 mRNA sequence of the rat and mouse homology region, we synthesized two pairs of complementary oligonucleotide strands . Double strands were formed after annealing, and then inserted into Pgenesil-1 plasmid expression vector. After identification by enzyme cutting and sequencing, the recombinant plasmids named P-Cx43-shRNA (1), P-Cx43-shRNA (2) and P-con-shRNA were transfected into the NIH/3T3 cells. Immunofluorescence and Western blot assays were used to detect the protein level of Cx43 after being screened by G418.The results of enzyme cutting and sequencing showed that we successfully constructed two shRNA expression vectors targeting Cx43, and a control expression vector for rat and mouse. Also, the Cx43 protein level was decreased by 73.5% ( < 0.01) and 10.8%, accordingly. The Cx43 protein level was not influenced by the transfection of P-con-shRNA. The outcomes demonstrate that the plasmid P-Cx43-shRNA (1) can specifically silence better the expression of Cx43 in NIH/3T3 cells, which offers an experimental evidence for further investigation.

关键词： RNA interference     connexin 43     small hairpin RNA (shRNA)     acupuncture    

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Transgenic technologies in cassava for nutritional improvement and viral disease resistance: a key strategy for food security in Africa

Maliwan NACONSIE,Peng ZHANG

《农业科学与工程前沿（英文）》 2016年 第3卷 第4期   页码 285-294 doi: 10.15302/J-FASE-2016119

摘要： As a major staple food source in Africa and other tropical developing countries, cassava ( ) provides basic sustenance for many subsistence farmers. However, cassava roots mainly accumulate starch with limited contribution of other nutrients such as proteins and vitamins. Also, two viral diseases, cassava mosaic disease (CMD) and cassava brown streak disease (CBSD), cause great losses in cassava production in sub-Saharan Africa and the Indian sub-continent. Genetic engineering provides promising approaches to improve nutritional value and increase resistance to viral diseases in cassava. This report presents several successful case studies on engineering protein content by overexpression of nutritious storage proteins and improving cassava resistance to viral diseases by RNA interference. Perspectives on the sustainable acquisition of new knowledge and development of biotechnology to solve these bottlenecks are discussed.

关键词： Manihot esculenta     protein enhancement     RNA interference     transgenesis     virus resistance    

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Ribozyme and the mechanisms that underlie RNA catalysis

Timothy J. Wilson,Yijin Liu,David M. J. Lilley

《化学科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 178-185 doi: 10.1007/s11705-016-1558-2

摘要： Ribozymes are widespread, and catalyze some extremely important reactions in the cell. Mechanistically most fall into one of two classes, using either metal ions or general acid-base catalysis. The nucleolytic ribozymes fall into the latter class, mostly using nucleobases. A sub-set of these use a combination of guanine base plus adenine acid to catalyze the cleavage reaction. New ribozymes are still being discovered at regular intervals and we can speculate on the potential existence of ribozymes that catalyze chemistry beyond phosphoryl transfer reactions, perhaps using small-molecule coenzymes.

关键词： RNA catalysis     RNA structure     catalytic mechanism    

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Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

《医学前沿（英文）》 2009年 第3卷 第3期   页码 287-291 doi: 10.1007/s11684-009-0050-5

摘要： In order to explore the role of cyclin-dependent kinase 4 (cdk4) in neurodegenerative diseases, lentiviral-delivered RNA interference (RNAi) was used to silence the expression of the murine cdk4 gene . Three cdk4-shRNAs of mouse and a negative sequence were designed. After synthesis and annealing, double strand oligonucleotides were cloned into a linearized pSIH1-H1-copGFP shRNA vector. It was confirmed by polymerase chain reaction (PCR) and sequencing that three pairs of cdk4-shRNAs and a negative shRNA were correctly inserted into the pSIH1-H1-copGFP vector. The above recombinants were transfected by lipofectamine into BV-2 cells. The gene silencing efficacy rates of the 3 targets were compared by Western blotting. The cdk4-siRNA2 was the most effective in silencing cdk4. The optimized pSIH1-cdk4-siRNA2 and pSIH-negative-siRNA were co-transfected into 293T cells with the lentiviral packaging plasmids respectively. The culture supernatant was harvested and condensed at the 24th and 48thh after transfection. Interference efficiency of the lentivirus expressing cdk4-siRNA was determined by reverse transcriptase-PCR (RT-PCR) and Western blotting in BV-2 cells. Lentivector-mediated RNAi could efficiently down-regulate the expression of the murine cdk4 gene , which provides a potential tool for studying and treating cdk4-related diseases.

关键词： cyclin-dependent kinase 4     RNA interference     plasmid     lentiviral vector    

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Elastoplastic frictional contact problem study on interference fits of compressor

ZHANG Hongwu, WU Changhua, LIAO Aihua

《能源前沿（英文）》 2008年 第2卷 第3期   页码 313-319 doi: 10.1007/s11708-008-0026-1

摘要： The FE parametric quadratic programming (PQP) method developed from the parametric variational principle (PVP) was used for the analysis of the stress distribution of the 3D elastoplastic frictional contact of an impeller-shaft sleeve-shaft. A locomotive-type turbocharger compressor with 24 blades under combined centrifugal and interference-fit loading was considered in the numerical analysis. The solution of elastoplastic frictional contact problems belongs to unspecified boundary problems where the interaction between two kinds of nonlinearities should occur. To save time in the numerical computation, a multi-substructure technique was adopted in the structural modeling. The effect of fit tolerance, wall thickness of the shaft sleeve, and rotational speed on the contact stress was discussed in detail in the numerical computation. To decrease the difficulty of the assembly process and ensure the safety of the working state, the amount of interference between the shaft sleeve and shaft by press-fitting should be controlled strictly to avoid the rapid increase of contact stress. The numerical results show that the algorithm has high accuracy and good convergence. The study can be referred to in deciding the proper fit tolerance and improving the design and manufacturing technology of compressor impellers.

关键词： computation     thickness     interference-fit     interference     unspecified    

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Laser interference fabrication of large-area functional periodic structure surface

Lei WANG, Zi-Han WANG, Yan-Hao YU, Hong-Bo SUN

《机械工程前沿（英文）》 2018年 第13卷 第4期   页码 493-503 doi: 10.1007/s11465-018-0507-9

摘要：

Functional periodic structures have attracted significant interest due to their natural capabilities in regulating surface energy, surface effective refractive index, and diffraction. Several technologies are used for the fabrication of these functional structures. The laser interference technique in particular has received attention because of its simplicity, low cost, and high-efficiency fabrication of large-area, micro/nanometer-scale, and periodically patterned structures in air conditions. Here, we reviewed the work on laser interference fabrication of large-area functional periodic structures for antireflection, self-cleaning, and superhydrophobicity based on our past and current research. For the common cases, four-beam interference and multi-exposure of two-beam interference were emphasized for their setup, structure diversity, and various applications for antireflection, self-cleaning, and superhydrophobicity. The relations between multi-beam interference and multi-exposure of two-beam interference were compared theoretically and experimentally. Nanostructures as a template for growing nanocrystals were also shown to present future possible applications in surface chemical control. Perspectives on future directions and applications for laser interference were presented.

关键词： laser interference     four-beam interference     multi-exposure of two-beam interference     additive fabrication    

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抗RNA病毒相关生物材料

姚康德,尹玉姬,张宝连,赵立国

《中国工程科学》 2003年 第5卷 第7期   页码 17-23

摘要：

在介绍RNA病毒结构、生殖、复制和转录的基础上，综述了抗病毒策略，其中包括抗SARS药物设计，RNA干扰，DNA疫苗释放系统，调控蛋白与糖胺聚糖衍生物或类似物相互作用，天然药物及肺泡组织工程等。这些实例涵盖了RNA病毒与蛋白质、DNA及多糖等生物材料的相互作用。生物材料作为基质或载体正在向细胞或/和基因活化的第三代生物材料发展，可望在抗病毒中发挥作用。

关键词： 抗RNA病毒     蛋白质     DNA     多糖     生物材料    

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敲低特异性环状非编码RNA显著抑制骨肉瘤的进展 Article

王世东, 张红亮, 李博, 陈成龙, 任婷婷, 黄怡, 刘凯, 李敬敬, 郭卫

《工程（英文）》 2023年 第21卷 第2期   页码 187-193 doi: 10.1016/j.eng.2021.12.007

摘要： 异常表达的非编码环状RNA（circRNA）对骨肉瘤的发生和发展至关重要。本研究的目的是探索一种新的circRNA circ_000203 在骨肉瘤中的表达和作用，阐明其潜在机制。

关键词： 非编码RNA     骨肉瘤     circRNA     分子机制     敲低    

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with multi-walled carbon nanotubes: preparation, characterization, and application for electromagnetic interference

Jian LI,Caichao WAN

《农业科学与工程前沿（英文）》 2015年 第2卷 第4期   页码 341-346 doi: 10.15302/J-FASE-2015082

摘要： Electromagnetic wave pollution has attracted extensive attention because of its ability to affect the operation of electronic machinery and endanger human health. In this work, the environmentally-friendly hybrid aerogels consisting of cellulose and multi-walled carbon nanotubes (MWCNTs) were fabricated. The aerogels have a low bulk density of 58.17 mg·cm . The incorporation of MWCNTs leads to an improvement in the thermal stability. In addition, the aerogels show a high electromagnetic interference (EMI) value of 19.4 dB. Meanwhile, the absorption-dominant shielding mechanism helps a lot to reduce secondary radiation, which is beneficial to develop novel eco-friendly EMI shielding materials.

关键词： cellulose aerogels     carbon nanotubes     electromagnetic interference shielding     composites    

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Electromagnetic interference modeling and suppression techniques in variable-frequency drive systems

Le YANG, Shuo WANG, Jianghua FENG

《机械工程前沿（英文）》 2018年 第13卷 第3期   页码 329-353 doi: 10.1007/s11465-018-0466-1

摘要：

Electromagnetic interference (EMI) causes electromechanical damage to the motors and degrades the reliability of variable-frequency drive (VFD) systems. Unlike fundamental frequency components in motor drive systems, high-frequency EMI noise, coupled with the parasitic parameters of the trough system, are difficult to analyze and reduce. In this article, EMI modeling techniques for different function units in a VFD system, including induction motors, motor bearings, and rectifier-inverters, are reviewed and evaluated in terms of applied frequency range, model parameterization, and model accuracy. The EMI models for the motors are categorized based on modeling techniques and model topologies. Motor bearing and shaft models are also reviewed, and techniques that are used to eliminate bearing current are evaluated. Modeling techniques for conventional rectifier-inverter systems are also summarized. EMI noise suppression techniques, including passive filter, Wheatstone bridge balance, active filter, and optimized modulation, are reviewed and compared based on the VFD system models.

关键词： variable-frequency drive (VFD)     electromagnetic interference (EMI)     motor drive modeling     EMI noise suppression    

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Experimental study of aerodynamic interference effects on aerostatic coefficients of twin deck bridges

Zhiwen LIU , Zhengqing CHEN , Gao LIU , Xinpeng SHAO ,

《结构与土木工程前沿（英文）》 2009年 第3卷 第3期   页码 292-298 doi: 10.1007/s11709-009-0048-8

摘要： The aerodynamic interference effects on aerostatic coefficients of twin deck bridges with large span were investigated in detail by means of wind tunnel test. The distances between the twin decks and wind attack angles were changed during the wind tunnel test to study the effects on aerodynamic interferences of aerostatic coefficients of twin decks. The research results have shown that the drag coefficients of the leeward deck are much smaller than that of a single leeward deck. The drag coefficients of a windward deck decrease slightly compared with that of a single deck. The lift and torque coefficients of windward and leeward decks are also affected slightly by the aerodynamic interference of twin decks. And the aerodynamic interference effects on lift and torque coefficients of twin decks can be neglected.

关键词： twin decks     aerodynamic interference effects     aerostatic coefficients     wind tunnel test    

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Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages

LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao

《医学前沿（英文）》 2008年 第2卷 第2期   页码 166-170 doi: 10.1007/s11684-008-0030-1

摘要： This study aims to demonstrate that blocking the receptor-interacting protein2 (Rip2) expression can decrease inflammatory cytokine production by macrophage and protect mice from endotoxin lethality. Murine Rip2 small interfering RNA (siRNA) plasmids were constructed and transfected into macrophage and Rip2 expression was detected with reverse transcription-polymerase chain reaction (RT-PCR) and western blot. Cell proliferation was assayed with MTT. TNF-? concentration was assayed with ELISA and high-mobility group box 1 protein (HMGB1) level with semi-quantitative western blot after lipopolysaccharide (LPS) stimulation. LPS challenge was given after the plasmids were injected into mice and the survival rate was calculated. Rip2 siRNA plasmid could block the mRNA and protein expression of Rip2 and promote cell proliferation. Blocking Rip2 could attenuate LPS-induced TNF-? and HMGB1 production. The HMGB1 expression in the liver decreased to (40.21 ± 11.03) pg/g, and serum TNF-? level decreased to (300.43 ± 59.26) ng/L ( < 0.05). The survival rate of mice from endotoxemia was also improved ( < 0.05). The results demonstrate that Rip2 siRNA plasmid can block the expression of Rip2, decrease the production of TNF-? and HMGB1 and protect mice from fatal endotoxemia.
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标题 作者 时间 类型 操作

Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene

Zhengjuan LIU, Jie BIAN, Yuchuan WANG, Yongli ZHAO, Dong YAN, Xiaoxia WANG

期刊论文

Effects of RNA interference targeting angiotensin 1a receptor on blood pressure and cardiac hypertrophy

ZHANG Jingqun, SUN Honglei, MA Yexin, WANG Daowen

期刊论文

基于RNA的生物防治——一种作物保护新模式

Matthew Bramlett, Geert Plaetinck, Peter Maienfisch

期刊论文

Gene silencing efficiency of shRNA expression vectors targeting Cx43

Cuihong ZHENG, Yunxia WU, Guangying HUANG, Wei WANG

期刊论文

Transgenic technologies in cassava for nutritional improvement and viral disease resistance: a key strategy for food security in Africa

Maliwan NACONSIE,Peng ZHANG

期刊论文

Ribozyme and the mechanisms that underlie RNA catalysis

Timothy J. Wilson,Yijin Liu,David M. J. Lilley

期刊论文

Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

期刊论文

Elastoplastic frictional contact problem study on interference fits of compressor

ZHANG Hongwu, WU Changhua, LIAO Aihua

期刊论文

Laser interference fabrication of large-area functional periodic structure surface

Lei WANG, Zi-Han WANG, Yan-Hao YU, Hong-Bo SUN

期刊论文

抗RNA病毒相关生物材料

姚康德,尹玉姬,张宝连,赵立国

期刊论文

敲低特异性环状非编码RNA显著抑制骨肉瘤的进展

王世东, 张红亮, 李博, 陈成龙, 任婷婷, 黄怡, 刘凯, 李敬敬, 郭卫

期刊论文

with multi-walled carbon nanotubes: preparation, characterization, and application for electromagnetic interference

Jian LI,Caichao WAN

期刊论文

Electromagnetic interference modeling and suppression techniques in variable-frequency drive systems

Le YANG, Shuo WANG, Jianghua FENG

期刊论文

Experimental study of aerodynamic interference effects on aerostatic coefficients of twin deck bridges

Zhiwen LIU , Zhengqing CHEN , Gao LIU , Xinpeng SHAO ,

期刊论文

Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages

LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao

期刊论文